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Peripheral intravenous administration of T cells sensitized to ovalbumin protects animals from experimental airway hyperreactivity. We have investigated whether protection is mediated by T cells recognizing the same or different epitopes of antigen to which the sensitized T cells respond. The expression of both Ly-2 and OX-8 on the cells of a sensitized adoptive transfer line was investigated by flow cytometry. When adoptively transferred, Ly-2+ or OX-8+ cells selectively protected naive rats from airway hyperreactivity. The protection mediated by Ly-2+ or OX-8+ T cells was inhibited if the cells of the adoptive transfer line were depleted of particular subclasses of T cells by adsorption with monoclonal antibodies. Protection by Ly-2+ T cells was shown to be mediated by Ly-2+ cells and to be specific for the T cells recognizing the same epitopes as the sensitized cells, because protection was inhibited by absorption of the T cells with a monoclonal antibody (anti-I-A) to which the sensitized T cells bore receptors. By contrast, protection by OX-8+ cells appeared to be mediated by OX-8+ T cells, and the protection by these cells was not inhibited by absorption with anti-I-A. These results show that different forms of protection can be mediated by cells recognizing the same or different epitopes of the antigen. They also demonstrate that OX-8+ T cells can mediate protection in an antigen-specific manner, and they raise the possibility that protection may be mediated by different functions in T cells recognizing the same antigen.Q:
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